Sure! Weird that it is only happening on my machine.
I can’t see where the article it keeps pulling is referenced in the JSON.
For clarity, the reference is: 10.1038/s41564-023-01558-w
and it keeps pulling the article pdf for: 10.1038/s41576-019-0159-6
In my connection, the paper I want is behind a paywall and I probably cannot access it - this is how I spotted that it was grabbing the wrong file.
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"url:https://www.nature.com/articles/s41564-023-01558-w",
"url:https://www.nature.com/articles/s41564-023-01558-w.pdf",
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"url:https://dx.doi.org/10.1038/s41564-023-01558-w"
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"published": {
"month": "1",
"year": "2024"
},
"affiliation": "Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA. ibrito@cornell.edu.",
"abstract": "Bacteria respond to environmental stimuli through precise regulation of transcription initiation and elongation. Bulk RNA sequencing primarily characterizes mature transcripts, so to identify actively transcribed loci we need to capture RNA polymerase (RNAP) complexed with nascent RNA. However, such capture methods have only previously been applied to culturable, genetically tractable organisms such as E. coli and B. subtilis. Here we apply precision run-on sequencing (PRO-seq) to profile nascent transcription in cultured E. coli and diverse uncultured bacteria. We demonstrate that PRO-seq can characterize the transcription of small, structured, or post-transcriptionally modified RNAs, which are often absent from bulk RNA-seq libraries. Applying PRO-seq to the human microbiome highlights taxon-specific RNAP pause motifs and pause-site distributions across non-coding RNA loci that reflect structure-coincident pausing. We also uncover concurrent transcription and cleavage of CRISPR guide RNAs and transfer RNAs. We demonstrate the utility of PRO-seq for exploring transcriptional dynamics in diverse microbial communities.",
"title": "Precision run-on sequencing (PRO-seq) for microbiome transcriptomics",
"pages": "241-250",
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"issn": "2058-5276",
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Thank you for your help!